Abstract: Objectives　To explore the clinical value of procalcitonin (PCT) in conjunction with heart-related markers in assessment of the severity of sepsis. Methods The clinical data of 147 children with sepsis (88 cases of sepsis and 59 cases of severe sepsis) admitted to pediatric intensive care unit (PICU) from September 2013 to April 2014 were retrospectively analyzed. The levels of procalcitonin and heart-related markers at different stages of sepsis were compared. Results The levels of PCT, CK-MB, NT-proBNP, and cTnT-hs in severe sepsis group were significantly higher than those in sepsis group (P<0.01). However, there was no significant difference in WBC and CRP between the two groups (P>0.05). Compared with surviving patients, the levels of PCT, CK-MB, cTnT-hs, and NT-proBNP were significantly higher in dead patients (P<0.05). The area under the curve (AUC) of PCT, CK-MB, NT-proBNP, and cTnT-hs in assessment of the severity of sepsis was 0.838, 0.811, 0.854 and 0.713, respectively. The AUC of PCT, CK-MB, NT-proBNP, and cTnT-hs in predicting survival situation was 0.766, 0.715, 0.778 and 0.609, respectively. A binary logistic regression equation was established based on the results of procalcitonin and heart-related indicators: logt(P) = 2.668×NT-proBNP+1.083×PCT+ 1.620×CK-MB+0.864×cTnT-hs–11.461. The AUC of logt (P) in assessment of the severity of sepsis and in predicting survival situation was 0.928 and 0.807. Conclusions Individual indicator of PCT, CK-MB, NT-proBNP, and cTnT-hs can be independently used to evaluate the severity of sepsis in children, and in comparison with single index, the assessment value is higher when four indicators are combined. The severity of sepsis in children is closely related to myocardial injury and cardiac dysfunction.